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Co-overexpression of TcpH increases the stability of TcpP. Western blot analysis of the ΔtcpP tcpH+ strain transformed with tcpP alone (ΔtcpP + P) expressed from the inducible plasmid tcpP (pBAD18) or cotransformed with tcpP(pBAD18) and tcpH (ΔtcpP + P + H) expressed from pACYC184. Rifampin was added to stop transcription of mid-log cultures. Cultures remained at 30°C after the addition of rifampin.
