FIG. 6.

Detachment of 18-h-old biofilm colonies of A. pleuropneumoniae strain IA5 grown in a 96-well microtiter plate. Biofilm colonies were treated with PBS with or without A. pleuropneumoniae (App) or A. actinomycetemcomitans (Aa) dispersin B (A); 50 mM sodium acetate (NaOAc) buffer, pH 4.8, with or without 10 mM sodium metaperiodate (B); 10 mM Tris-HCl (pH 7.6)-100 mM NaCl with or without 10 μg of proteinase K/ml (C). (D) Untreated controls. Biofilms were stained with crystal violet as previously described (16).