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. 2017 Feb 24;7:43306. doi: 10.1038/srep43306

Table 1. Strains and plasmids constructs.

Strain/plasmids Relevant genetic marker (s) or features Sources/reference
E. coli Strains
Mach-I ΔrecA1398 endA1 tonA Φ80ΔlacM15 ΔlacX74 hsdR (rK− K+) Invitrogen
C43 (DE3) F− ompT gal dcm hsdSB (rB− mB−) (DE3) 56
Lemo21 (DE3) fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/pLemo (CamR) New England Biolabs
Plasmids
pDML924 pET28a, His-ponB 50
pDML924 (S510A) pET28a, His-ponB (S510A) This work
pDML2032 pET22b, ftsN-His This work
pDML2422 pMCL210-ftsW-293HA 18
pDML2400 pET28a, His-ftsW 18
pDML2040 pETDuet, His-ftsI, ftsW This work
pDML2041 pETDuet, His-ftsW, ftsI This work
pDML2043 pETDuet, His-ftsI, ftsW (293HA) This work
pDML2045 pACYCDuet, ponB, ftsN-S. tag This work
pCIP1000 pETDuet, His-ftsW, ponB This work
pCIP1083 pETDuet, His-ftsWHA, ponB This work
pET28MHL-WSe pET28MHL, His-ftsWSe This work
pET28MHL-WKp pET28MHL, His-ftsWKp This work
pCIP1051 pETDuet, His-murJ This work