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. 2017 Feb 24;7:43166. doi: 10.1038/srep43166

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) A tree of expression correlations between samples. Correlations were calculated using the expression levels of lncRNAs in 11 tissue samples (9 organs and 3 developmental stages of skeletal muscle). (B) The tissue-specificity scores of novel lncRNAs compared with those of annotated lncRNAs and protein-coding genes. (C) Heat map of the expression of tissue-specific lncRNAs. (D) qPCR validation of the expression levels in 9 tissues for 8 randomly selected S. scrofa lncRNAs (blue bar and Y-axis on the right). Error bars indicate standard deviation (SD) based on three biological replicates. The RPKM values of the lncRNAs from RNA-seq data were also shown (red line and Y-axis on the left).