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. 2017 Feb 24;7:43168. doi: 10.1038/srep43168

Figure 5. A mitochondrial amplification loop promotes ripoptosome formation.

Figure 5

(A) CEMNeo or CEMBclXL cells were treated with DMF (50 μM), PMX464 (PMX, 1 μM), LCL161 (LCL, 20 μM), or etoposide (Eto, 50 μM) for 8 h (red). DMSO was used as vehicle control (black). CCCP was used as positive control (grey). MOMP was assessed by staining with TMRE. (B) Specific cell death was analyzed in cells stimulated with DMF (50 μM), PMX464 (PMX, 1 μM), LCL161 (LCL, 20 μM), or etoposide (Eto, 50 μM) for 18 h (n = 3). (C) CEMNeo or CEMBclXL cells were treated with DMSO (Veh), DMF (50 μM), etoposide (Eto, 50 μM), or LCL161 (LCL, 20 μM) for 18 h. Lysates were subjected to Western blot and analyzed for caspase-3 (C3), −9 (C9), −8 (C8), RIPK1 and Tubulin. (D) Mitochondrial amplification loop promotes ripoptosome formation. Cells were pre-incubated with zVad and Nec-1, then stimulated as in (C) for 24 h. IP was performed with antibodies against caspase-8 (α-C8). (B) Error bars represent standard deviation.