Table 2. Comparison of cream separator and conventional sucrose method for the determination of maximal and minimal recovery rates of eimerian oocysts and nematode eggs.
| Description | Cream separator method |
Sucrose gradient method |
||
|---|---|---|---|---|
| Eimerian oocysts | Trichostronglyid eggs | Eimerian oocysts | Trichostronglyid eggs | |
| Average maximal recovery ( ± SD)a | 80.68% ± 1.61% | 91.91% ± 1.35% | 81.87% ± 9.81% | 92.32% ± 3.13% |
| Average minimal egg recovery number ( ± SD)a (3 oocysts/eggs per ml of applied faecal material suspension) | 0.9% ± 0.74% | 1.6% ± 0.55% | 1% ± 1.03% | 1.2% ± 0.8 4% |
| Viability ratea | 68.06% ± 1.9% | 73.74% ± 7.61% | 69.75% ± 1.47% | 74.164% ± 1.648% |
| Sample size | 960 g | 0.6 g (classical sucrose gradient set up) and 1.2 g (alternative sucrose gradient set up) | ||
| Capacity | 12 liter of faecal suspension (MOTOP C14-100, Ukraine) | 7.5 ml of faecal suspension (classical sucrose gradient set up)/15 ml (alternative sucrose gradient set up)(Falcon™ 50 ml conical centrifuge tubes) | ||
| Time required | 7.2 min ± 0.8 min | 15 min ± 2.05 min | ||
aEach value is a mean of ten separate runs.