TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| S. aureus strains | ||
| SAM23 | 8325-4 | 50 |
| SAM13 | RN4220 (r− m+) | 33 |
| SAM1100 | SAM13/pMP797 | This study |
| E. coli DH5α | F−endA1 hsdR17 recA1 gyrA96 relA1 | Life Technologies, Inc. |
| Plasmids | ||
| pCL84 | S. aureus-E. coli shuttle vector; Tcr Spcr | 36 |
| pC194 | S. aureus plasmid; Cmr | Bacillus Genetic Stock Center |
| pT181 | S. aureus plasmid; Tcr | Bacillus Genetic Stock Center |
| pE194 | S. aureus plasmid; Ermr | Bacillus Genetic Stock Center |
| pYL112Δ19 | S. aureus plasmid carrying L54a int; Cmr | 36 |
| PMP16 | S. aureus-E. coli shuttle vector; Ermr Ampr | 41 |
| pMP202 | pBluescript KS(+)::tet(k); Ampr Tcr | This study |
| pMP797 | pMP16::int | This study |
| pMP820 | S. aureus-E. coli shuttle vector; Cmr Spcr | This study |
| pTV32ts | S. aureus Tn917lac delivery plasmid; Ermr | P. Youngman (unpublished) |
| pI258repA36 Ω1(Tn551) | S. aureus Tn551 delivery plasmid; Ermr | 47 |
| pBluescript KS(+) | E. coli cloning vector; Ampr | Stratagene, Inc. |