TABLE 2.

Attenuated S. aureus strains identified by SMIT

Classification	Strain	Gene (organism)a	Lengthb	Insertion sitec	Strain N315 ORF	Putative or known function
Biosynthesis of small molecules	IVT15	lysC	401	196	SA1225	Aspartate kinase
	IVT36	tyrA (B. halodurans)	366	263	SA1197	Prephenate dehydrogenase
	IVT173	dapB	230	132	SA1228	Dihydrodipicolinate synthase
	IVT252	hipO	383	367	SA1230	Hippurate hydrolyase
	IVT285	trpF	211	53	SA1203	Phosphoribosylanthranilate isomerase
	IVT626	aspB (B. circulans)	428	154	SA1749	Aspartate aminotransferase
	IVT627	pycA (B. halodurans)	1,073	589	SA0963	Pyruvate carboxylase
	IVT636	pyrC (B. halodurans)	425	131	SA1044	Dihydroorotase
	IVT638	asd	329	91	SA1226	Aspartate semialdehyde dehydrogenase
Cell surface binding and transport	IVT35	vga	642	512	SA1852	Streptogramin resistance ABC-type transporter
	IVT133	yflS (B. subtilis)	472	159	SA2486	Predicted 2-oxoglutarate/malate translocator
	IVT176	yuiF (B. subtilis)	438	46	SA0804	Predicted permease
	IVT207	braB	448	2	SA1239	Branched-chain amino acid transporter
	IVT291	pstS (B. anthracis)	327	15	SA1221	Phosphate ABC transporter binding protein
	IVT631	gabP	484	400	SA1169	Gamma-aminobutyrate permease
Signal transduction	IVT24	saeS	353	278	SA0660	Regulator of exotoxin gene expression
	IVT59	arlS	451	401	SA1246	Regulator of extracellular proteases
	IVT84	arlS	451	118	SA1246	Regulator of extracellular proteases
Energy	IVT40	narJ (S. carnosus)	200	151	SA2183	Anaerobic nitrate reductase, delta subunit
Unknown	IVT27	SE0526 (S. epidermidis)	164	161	SA0699
	IVT29	SE1059 (S. epidermidis)	255	251	SA1209
	IVT42	yitL (B. subtilis)	300	279	SA1223
	IVT171	LMO2587 (L. monocytogenes)	282	92	SA2478
	IVT211	yneP (B. subtilis)	155	113	SA1185	Predicted thioesterase

^aPreviously described S. aureus gene designation or name of the gene and source organism encoding the ORF with highest similarity.

^bGene ORF length (condons).

^cCodon site of transposon insertion.