TABLE 3.

Phenotypic and competitive virulence analysis of SMIT mutants

Strain	Disrupted gene or homolog	Apparent auxotropy	CIa
			In vitro	In vivo
IVT15	lysC	Lysine	0.84	0.32
IVT24	saeS		0.36	0.15
IVT27	ORF		0.31	0.11
IVT29	ORF		0.82	0.32
IVT35	ydiF		0.94	0.40
IVT36	tyrA	Tyrosine	0.79	0.28
IVT40	narJ		0.70	0.67
IVT42	yitL		0.29	0.25
IVT59	arlS		0.25	0.10
IVT133	yflS		1.06	0.14
IVT171	LMO2587		1.36	0.63
IVT173	dapB	Lysine	0.84	0.24
IVT176	yuiF		0.92	0.16
IVT207	braB	Lysine	0.84	0.29
IVT211	yneP		0.44	0.28
IVT252	hipO	Lysine	1.10	0.20
IVT285	trpF	Tryptophan	0.89	0.59
IVT291	phoX		0.92	0.67
IVT626	aspB	Aspartate	0.73	0.001
IVT627	pycA	Aspartate	0.91	0.02
IVT631	gabP		0.82	0.03
IVT636	pyrC	Uracil	0.34	0.19
IVT638	asd	Lysine	0.80	0.26

^aCI determinations were relative to isogenic wild-type SAM23 (see Materials and Methods).