Table 1. PRKCB promoter methylation in normal B and CLL cells.
| Sample ID | B-CLL/Normal B | % purity | Mean % methylation |
|---|---|---|---|
| 2649 | Normal B | 96.12 | 3.49 |
| 2667 | 97.12 | 2.22 | |
| 2668 | 93.15 | 2.3 | |
| 2675 | — | 1.73 | |
| 2063 | B-CLL | 99.79 | 3.32 |
| 2064 | — | 4.51 | |
| 2226 | — | 2.31 | |
| 2262 | 93.03 | 1.05 | |
| 2458 | 91.09 | 0.8 | |
| 2536 | 99.33 | 3.16 |
Normal B and CLL cells were purified each from 5 healthy donors or CLL patients, respectively. Genomic DNA was isolated from these cells, and methylation of the PRKCB promoter was determined as described in the materials and methods. % methylation is reported as the mean average methylation of 8 CpGs within the analyzed sequence. Where a dash occurs within the % purity of the cells indicates where cell purity was not assessed following purification.