Table 1. Primer sequences used for genotyping and exon PCR.

Genotyping reaction	Forward primer	Reverse Primer	Annealing temperature [°C]
Mapt wt	ttg aat ctc tcc ctg gac atg g	ttg tgt caa act cct ggc gag	60
Mapt ko	ttg aat ctc tcc ctg gac atg g	ctt cta tcg cct tct tga	55
PAC-MAPT junction 5′	atg gct cat aac acc cct tg	ggt atg ggg gtc att ttt cc	63
PAC-MAPT junction 3′	act gac ccc acc aaa cct c	caa tga cct gac cat ttg atg	63
Promoter/Exon-1	agg aac gag ccg gga gac	gac ggc gag gca gat ttc	64
Exon 1	tga gat ctg cct gcc atg aa	cat ggc tgt cca cta acc tt	58
Exon 2	ggc tca ctg tat gtg ttc ca	agc acc agc aag caa ggc at	58
Exon 3	cag ggc tgc ttt ctg gca ta	cag cag ggc ctt gac tgc ct	58
Exon 4	cct tca ttt gct gac aca ta	caa ggc tag cat ctt tca ga	58
Exon 4A	cac cac tgc gta tct cca ca	gaa cgt cag aag cag cag ga	58
Exon 5	cag ctg gct ttc tgt gaa ca	tca tga acc tgc caa ctg ct	58
Exon 6	ctc ctc cat gtg ctg act tt	ttg caa ac caca gca gag ca	58
Exon 7	gac tct tgg tgg cag taa ct	acc tct gag agc ttc agc tt	58
Exon 8	gcc acg tga agg act cat ta	caa act gca cag gga aga ga	58
Exon 9	ctg ctg tag ctg cgc ttc ca	ctc cat gca cag tcc cac ga	58
Exon 10	ctg cca agt ccg aaa gtg ga	aga tcc tga gag ccc aag aa	58
Exon 11	gct tac aca gct gct tct ca	cac ctt gtc ttg ggc agc at	58
Exon 12	gtc ttc ttc cct cca gag ca	tcc agc cag tca aca cag aa	58
Exon 13	gat tgt gcc acc gca ctc ta	cct gat cac aaa ccc tgc tt	58
Exon 14	tgc tcc aca gaa acc ctg tt	ctg caa cca acc agg gtc at	58
Saitohin (STH)	ccc tgt aaa ctc tga cca cac	aca ggg aag cta ctt ccc atg	58
238 bp indel	gga aga cgt tct cac tga tct g	agg agt ctg gct tca gtc tct c	58