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Northern blot analysis of transcriptional read-through from the ermB promoter. RNA from the pep27 mutant HS0602 and the vncR mutant HS0603 was isolated after the cells were grown in the absence or presence of erythromycin, and samples were subjected to Northern blot analysis with probes specific for ermB or vncS. High-molecular-weight bands represent ermB-vncR-vncS (filled arrow) or ermB-vncS (open arrow) polycistronic mRNAs. rRNA is shown as the loading control.
