FIG. 3.

Effect of the orientation of the ermB cassette on autolysis. Wild-type strain T4 (black line) served as the control for all panels. (A) Autolysis of the lytA autolysin mutants HS1937 (open squares) and HR1937 (filled squares). (B) Autolysis of the vncS histidine kinase mutants HS0604 (open diamonds) and HR0604 (filled diamonds). (C) Autolysis of the vex3 transmembrane protein mutants HS0601 (open circles) and HR0601 (filled circles). All samples were treated with 10× MIC of vancomycin as the cultures reached an OD₆₂₀ of 0.275 to 0.30. Means and standard deviations for three independent experiments are shown.