FIG. 7.

Transcriptional analysis of the gonococcal rpoH upstream region in N. gonorrhoeae strains JKD488 and JKD489, which contain promoter::cat transcriptional fusions integrated into the conjugative plasmid ptetM25.2. These strains carry the wild-type rpoH promoter region and that with the deleted leader sequence, respectively. Strain JKD487, containing a promoterless cat gene, was the negative control. Cell extracts were prepared from strains exposed to heat shock at 42°C for 20 min. The CAT levels shown are the means of results from four separate assays. The error bars represent standard deviations.