TABLE 2.
Translational parameters of σs synthesis of exponential- and stationary-phase NG and SMG culturesa
| Growth phase | σs proteinb [σs]
|
σs half-lifec (τ1/2σs)
|
Translational rated (Ksσs)
|
Translational efficiencye (KEσs)
|
||||
|---|---|---|---|---|---|---|---|---|
| NG | SMG | NG | SMG | NG | SMG | NG | SMG | |
| Exponential | 73 | 53 | 16.4 | 5.2 | 3.1 | 7.0 | 3.6 × 1011 | 8.4 × 1011 |
| Stationary | 1,263 | 2,530 | 28.8 | 24.6 | 30.4 | 71.2 | 3.3 × 1013 | 8.5 × 1013 |
a
Each value is an average of at least two independent determinations with analytical triplicates; standard error of the mean was <8%.
b
Nanograms σs per milligram of protein.
c
Protein half-life (minutes).
d
Nanograms of σs synthesized per milligram of cell protein per minute.
e
Molecules of σs synthesized per copy of rpoS mRNA per minute.