Table 2.

Effects of different supplements on the co-production of PMA and pullulan by A.pullulans HA-4D in JAT fermentation medium. Shake flask fermentation was carried out at 25 °C and 200 rpm for 7 days

Components	Concentration (g/L)	PMA (g/L)	Pullulan (g/L)	DCW (g/L)
NaNO3	0	31.9 ± 0.5	5.3 ± 0.4	12.5 ± 0.3
	1	33.2 ± 0.4*	6.3 ± 0.4	13.9 ± 0.4*
	2	30.8 ± 0.4	5.8 ± 0.4	15.8 ± 0.5**
KH2PO4	0	34.0 ± 0.4	6.1 ± 0.5	12.8 ± 0.4
	0.1	33.4 ± 0.4	6.3 ± 0.4	13.9 ± 0.9
	0.2	32.8 ± 1.1	6.6 ± 0.5	14.3 ± 0.8
MgSO4	0	35.1 ± 0.3	5.8 ± 0.5	13.2 ± 0.5
	0.1	34.1 ± 0.8	6.1 ± 0.3	12.9 ± 0.6
	0.2	33.7 ± 0.9	6.7 ± 0.3	12.5 ± 0.4
KCl	0	36.3 ± 0.5	5.5 ± 0.4	11.9 ± 0.2
	0.5	35.1 ± 0.5	5.7 ± 0.4	12.5 ± 0.4
	1	34.6 ± 0.9	6.4 ± 0.3	12.9 ± 0.8
ZnSO4	0	36.8 ± 0.4	6.0 ± 0.3	12.5 ± 0.4
	0.1	36.2 ± 0.6	5.5 ± 0.4	11.9 ± 0.4
	0.2	35.8 ± 0.4	5.1 ± 0.5	11.6 ± 0.3
CaCO3	0	26.3 ± 0.4	9.7 ± 0.6	11.5 ± 0.4
	15	33.9 ± 0.6**	6.8 ± 0.2**	11.9 ± 0.5
	30	36.6 ± 0.7**	6.0 ± 0.4**	12.4 ± 0.9

*0.01 < P < 0.05

**P < 0.01