TABLE 2.
Growth-phase-dependent expression of RpoS-dependent operon-lacZ fusions in strains grown in rich and minimal mediaa
| Strain | Gene | β-Galactosidase activity (Miller units) in:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LB
|
M9 glucose
|
||||||||||||
| Exponential phase
|
Stationary phase
|
Exponential phase
|
Stationary phase
|
||||||||||
| WT | rpoS mutant | RpoS dependent | WT | rpoS mutant | RpoS dependent | WT | rpoS mutant | RpoS dependent | WT | rpoS mutant | RpoS dependent | ||
| HS1002 | yjbJ | 6.2 | 3.2 | 2 | 74.4 | 3.4 | 22 | 20.2 | 3.6 | 6 | 53.7 | 0.3 | 180 |
| HS1006 | yjbE | 4.2 | 2.1 | 2 | 45.2 | 2.1 | 22 | 23.9 | 1.0 | 24 | 63.3 | 1.1 | 58 |
| HS1008 | aldB | 5.0 | 1.8 | 3 | 91.4 | 4.3 | 22 | 4.3 | 0.3 | 14 | 8.2 | 0.4 | 21 |
| HS1010 | gabP | 5.1 | 2.8 | 2 | 111 | 4.7 | 24 | 6.9 | 0.5 | 14 | 26.8 | 1.5 | 18 |
| HS1011 | ygaU | 15.4 | 18.9 | 1 | 144 | 33.5 | 4 | 58.9 | 6.9 | 9 | 198 | 16.0 | 12 |
| HS1012 | ygdI | 13.8 | 3.9 | 4 | 54.5 | 7.6 | 7 | 8.4 | 4.7 | 2 | 21.3 | 4.0 | 5 |
| HS1014 | katE | 12.7 | 1.9 | 7 | 80.3 | 2.0 | 40 | 23.4 | 0.3 | 78 | 79.4 | 1.2 | 66 |
| HS1019 | yqhE | 7.4 | 10.5 | 2 | 91.4 | 12.2 | 8 | 20.2 | 3.4 | 6 | 48.3 | 4.6 | 11 |
| HS1020 | mltB | 12.3 | 38.1 | 0.3 | 90.1 | 52.5 | 2 | 3.5 | 5.3 | 1 | 26.1 | 16.3 | 2 |
| HS1022 | narY | 3.4 | 2.5 | 1 | 28.3 | 3.0 | 10 | 0.9 | 1.2 | 1 | 5.4 | 2.4 | 2 |
| HS1024 | ygaF | 3.1 | 10.2 | 0.3 | 49.7 | 18.8 | 3 | 2.3 | 1.0 | 2 | 16.9 | 2.5 | 7 |
| HS1026 | ybaY | 29.8 | 10.1 | 3 | 243 | 16.4 | 15 | 16.1 | 1.5 | 11 | 132 | 11.0 | 12 |
| HS1028 | yjgR | 14.4 | 7.4 | 2 | 63.0 | 11.5 | 6 | 3.5 | 1.5 | 2 | 27.6 | 8.1 | 3 |
| HS1033 | ydaM | 8.6 | 0.9 | 10 | 35.2 | 2.2 | 16 | 20.8 | 2.1 | 10 | 38.2 | 1.5 | 25 |
| HS1035 | ydcS | 6.7 | 2.4 | 3 | 21.7 | 3.9 | 6 | 2.0 | 1.1 | 2 | 18.9 | 12.7 | 2 |
| HS1036 | ydcK | 0.7 | 1.8 | 0.4 | 46.2 | 3.0 | 15 | 5.2 | 1.9 | 3 | 26.7 | 3.1 | 9 |
| HS1037 | gabD-gabT | 0.7 | 0.6 | 1 | 11.0 | 1.3 | 8 | 1.9 | 0.3 | 6 | 3.7 | 0.6 | 6 |
| HS1042 | yhjY | 11.7 | 3.6 | 3 | 66.8 | 4.5 | 15 | 3.0 | 1.2 | 3 | 29.1 | 3.2 | 9 |
| HS1045 | yhiN | 16.1 | 11.1 | 1 | 60.1 | 13.0 | 5 | 12.7 | 1.6 | 8 | 16.3 | 2.5 | 7 |
| HS1049 | yeaG | 11.7 | 2.2 | 5 | 42.2 | 3.3 | 13 | 12.3 | 0.5 | 25 | 13.7 | 1.4 | 10 |
| HS1050 | phnP | 17.4 | 5.4 | 3 | 51.1 | 6.5 | 8 | 2.7 | 1.0 | 3 | 2.2 | 1.4 | 2 |
| HS1054 | yebF | 23.0 | 2.7 | 9 | 225 | 5.5 | 41 | 44.6 | 3.6 | 13 | 87.7 | 3.6 | 25 |
| HS1056 | yodC | 7.1 | 1.9 | 4 | 166 | 4.0 | 41 | 9.78 | 0.9 | 11 | 23.6 | 1.4 | 17 |
| HS1057 | gabD | 4.6 | 3.7 | 1 | 245 | 15.7 | 16 | 12.4 | 1.3 | 10 | 27.1 | 2.1 | 13 |
| HS1059 | talA | 3.2 | 3.3 | 1 | 106 | 5.3 | 20 | 13.3 | 0.9 | 15 | 42.6 | 1.6 | 26 |
| HS1061 | aroM | 2.1 | 2.1 | 1 | 74.8 | 5.0 | 15 | 1.81 | 0.3 | 6 | 31.9 | 0.4 | 78 |
| HS1063 | ugpE | 2.7 | 3.5 | 1 | 47.0 | 8.1 | 6 | 7.3 | 0.6 | 12 | 10.8 | 1.9 | 6 |
| HS1064 | nlpA-yicM | 5.9 | 7.8 | 1 | 30.7 | 11.1 | 3 | 8.8 | 6.1 | 1 | 23.1 | 6.0 | 4 |
| HS1066 | argH-oxyR | 12.3 | 14.8 | 1 | 30.0 | 18.9 | 2 | 88.3 | 81.4 | 1 | 102 | 102 | 1 |
| HS1067 | ybiO | 2.4 | 1.0 | 2 | 11.9 | 1.0 | 13 | 7.0 | 0.4 | 18 | 15.8 | 0.6 | 26 |
| HS1071 | yhjG | 12.8 | 6.0 | 2 | 159 | 9.4 | 17 | 27.7 | 1.8 | 15 | 95.7 | 6.3 | 15 |
| HS1072 | argH | 9.2 | 12.4 | 1 | 33.9 | 18.3 | 2 | 42.8 | 24.5 | 2 | 90.8 | 17.9 | 5 |
| HS1073 | yliI | 2.5 | 1.0 | 3 | 20.3 | 1.2 | 17 | 13.4 | 0.4 | 34 | 22.0 | 0.6 | 37 |
| HS1075 | ugpC | 2.7 | 1.3 | 2 | 26.0 | 1.3 | 20 | 3.2 | 0.7 | 5 | 9.9 | 1.1 | 9 |
| HS1077 | yhiV | 2.1 | 1.9 | 1 | 101 | 3.3 | 31 | 13.2 | 1.2 | 11 | 86 | 22.5 | 4 |
| HS1078 | uspB | 4.5 | 5.6 | 1 | 30.0 | 7.1 | 4 | 21.1 | 2.0 | 11 | 66.6 | 8.2 | 8 |
| HS1079 | ldcC | 4.9 | 3.4 | 1 | 29.8 | 4.5 | 7 | 18.1 | 1.3 | 14 | 46.6 | 4.3 | 11 |
| HS1080 | appB | 2.7 | 0.9 | 3 | 112 | 4.6 | 25 | 8.4 | 1.6 | 5 | 9.3 | 1.3 | 7 |
| HS1081 | yhiU | 6.0 | 2.1 | 3 | 112 | 2.9 | 39 | 34.0 | 2.5 | 14 | 117 | 14.0 | 8 |
| HS1082 | aidB | 8.8 | 3.0 | 3 | 138 | 3.8 | 36 | 22.2 | 1.0 | 20 | 111 | 3.4 | 33 |
| HS1084 | yehX | 3.6 | 1.5 | 2 | 58.3 | 2.1 | 28 | 23.1 | 1.0 | 23 | 55.5 | 2.6 | 21 |
| HS1090 | yphA | 6.0 | 2.7 | 2 | 64.5 | 2.7 | 24 | 25.2 | 1.0 | 25 | 67.5 | 3.7 | 18 |
| HS1091 | osmY | 15.9 | 6.0 | 3 | 264 | 7.5 | 35 | 110 | 2.3 | 48 | 210 | 3.3 | 64 |
| HS1092 | yfcG | 3.3 | 1.2 | 3 | 62.3 | 1.7 | 37 | 16.3 | 0.6 | 27 | 67.9 | 1.3 | 52 |
| HS1094 | otsA | 8.5 | 3.1 | 3 | 165 | 5.4 | 31 | 80.1 | 2.3 | 35 | 159.8 | 2.8 | 57 |
| HS1095 | ecnB | 15.0 | 7.3 | 2 | 144 | 6.3 | 23 | 48.9 | 1.9 | 26 | 134.0 | 1.8 | 74 |
| HS1099 | yhjD | 29.2 | 37.9 | 1 | 172 | 33.6 | 5 | 47.3 | 20.9 | 2 | 87.4 | 9.5 | 34 |
| HS1100 | yjiN | 8.6 | 6.2 | 1 | 66.8 | 15.6 | 4 | 30.2 | 4.0 | 8 | 52.2 | 9.5 | 6 |
a
Strains were grown overnight in LB broth, subcultured, and maintained in the appropriate media for eight generations prior to sampling. Cultures were sampled at exponential phase (OD600 was 0.3 for rich media and 0.2 for minimal media) and stationary phase (OD600 was 1.5 for rich media and 1.3 for minimal media). Cultures of strain HS1072 grown in M9 glucose medium were supplemented with 20 μg of arginine/ml. Values are the averages of three independent experiments, and the coefficient of variation was less than 10%.