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. 2017 Feb 24;18:10. doi: 10.1186/s12865-017-0193-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SDS-PAGE (a) and immunoblot (b and c) of cat epithelia in reducing conditions (15%T-2.67%C): Precision Plus Protein Dual Extra Standard (lane 1), NE (100 μg extract, lane 2) and CDA (100 μg, lane 3). Immunoblots were performed using serum from cat sensitized patients (b) or monoclonal antibody α-Fel d 1 (c) as primary antibody. High molecular weight SDS (d): HiMark^TM Pre-Stained HMW Protein Standard (lane 1), CDA (100 μg extract, lane 2), and NE (100 μg, lane 3)