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SDS-PAGE analysis of cell extracts from L. mesenteroides and S. mutans strains. (A) Zymogram of active glucansucrases after SDS-PAGE separation and incubation in sucrose. (B) The same samples electroblotted and developed with biotin-dextran to detect glucan binding proteins. Lane 1, L. mesenteroides NRRL B-1355; lane 2, L. mesenteroides NRRL B-512F; lane 3, L. mesenteroides NRRL B-1299; lane 4, S. mutans UA159; lane M, molecular mass ladder of 250, 150, and 100 kDa (arrows).
