Fig. 5.

Microbiota depletion, by antibiotic treatment or IMD activation, leads to SINV particles increase in Ae. aegypti. After 4 days post-infection the amount of viral RNA and mature particles were measured using mosquito whole body samples. Mosquitoes were maintained in normal sugar or pretreated with antibiotic solution before feeding an infectious blood meal. a Viral RNA amounts relative to the reference gene (RP49). Mosquitoes maintained in normal sugar and then infected with SINV were set as 1. b Plaque assay of whole body samples from mosquitoes infected with SINV 7 days after infection. Mosquitoes injected with dsLacZ-control and dsCaspar were infected with SINV and the levels of bacteria and virus were measured by qPCR. c Four days post-infection the 16S expression was measured in pools of five mosquitoes. d After 7 days post-infection, SINV RNA was measured in whole body of individual mosquitoes. We calculated the relative expression of both bacteria and virus using the LacZ-control condition as a reference. Statistical analysis were conducted using Student’s t-test (unpaired)