FIG. 1.
Schematic representation of HMW1 and recombinant HMW1 constructs. (A) HMW1 with the sequence of the C-terminal 112 amino acids shown above and paired Ser residues underlined. Regions affected by deletion are represented by patterned boxes. The numbers above each correspond to the four paired Ser residues. Amino acid positions corresponding to each region are indicated below the diagram, and the relative position of the BamHI site is shown for reference (not to scale). (B) HMW1 deletion derivatives, with protein designation indicated to the right for each. (C and D) Schematic of relevant regions of plasmids pKV74 and pKV37 (not to scale). Restriction sites: E, EcoRI; B, BamHI; K, KpnI; Gmr, gentamicin resistance gene.