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. 2004 Dec;186(24):8221–8228. doi: 10.1128/JB.186.24.8221-8228.2004

FIG. 2.

FIG. 2.

(A) Western immunoblot analysis of HMW2 and P1 steady-state levels in wild-type and mutant M6 M. pneumoniae. Mycoplasma samples at the indicated protein concentrations were subjected to SDS-PAGE and Western immunoblotting with anti-HMW2 serum (1:1,000) and anti-P1 serum (1:1,000). Lanes: WT, 75, 25 or 12.5 μg of wild-type M. pneumoniae protein, as indicated; M6, 75 μg of protein from mutant M6; M6 + reHMW1, 75 μg of M6 transformant producing full-length recombinant HMW1. Arrowheads indicate HMW2 and P1, while the 200-kDa protein mass marker is shown to the left. (B) Pulse-chase analysis of HMW2 synthesis and turnover in wild-type (WT), mutant M6, and mutant I-2 M. pneumoniae. The arrowheads indicate HMW1, HMW2, pre-P1, and mature P1; time points are given at the top in hours.