FIG. 5.

Western immunoblot analysis of the stability of HMW1 deletion derivatives in mutant M6 M. pneumoniae. Two independent transformants are shown for each HMW1 derivative. Equal amounts of protein were loaded per lane, separated by electrophoresis, transferred to nitrocellulose, and probed with anti-HMW1 serum (1:10,000). WT, untransformed wild-type M. pneumoniae; M6, untransformed mutant M6; M6 +, M6 M. pneumoniae transformants producing the indicated HMW1 derivative. The large arrowhead indicates HMW1, while smaller arrowheads indicate recombinant HMW1 derivatives.