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PCR primers used for construction of HMW1 deletion derivatives
| Plasmid | HMW1 derivative | Upstream primera | Downstream primera | Nucleotides amplifiedb |
|---|---|---|---|---|
| pKV205 | HMW1 Δ4 | GGATGGCGAAGCTAGACAG | ATGGATCCTTATGGCTCTAAC | 2666-2942 |
| pKV219 | HMW1 Δ1 | TGAGGATCCCTTGGACTTCTCC | GGCGGGATCCTTTGAAATACTACC | 2778-3116 |
| pKV213 | reHMW1 | GGATGGCGAAGCTAGACAG | GGCGGGATCCTTTGAAATACTACC | 2666-3116 |
Altered nucleotides are shown in boldface.
Numbers indicate position within the hmw1 coding sequence (9).
