Table 2.
Primers used in the real-time quantitative PCR assay of gene transcription
| Gene name | Accession number | Primer sequences (5′-3′) | Annealing temperature (°C) | PCR product (bp) |
|---|---|---|---|---|
| β-actin | L08165.1 | upstream: TGACGCAGATCATGTTTGAGA | 60 | 159 |
| downstream: GCAGAGCGTAGCCCTCATAG | ||||
| GnRH-I | EF495207.1 | upstream: CTGGGACCCTTGCTGTTTTG | 60 | 232 |
| downstream: AGGGGACTTCCAACCATCAC | ||||
| GnIH | KC514473.1 | upstream: ATCTACCTAGGCATGCTCCAA | 58 | 115 |
| downstream: ACAGGCAGTGACTTCCCAAAT | ||||
| VIP | DQ023159 | upstream: ACCAGTGTCTACAGCCATCTTTTG | 58 | 204 |
| downstream: AGGTGGCTCAGCAGTTCATCTACA | ||||
| TRH | NM_001030383.2 | upstream: GCAAGAGGGGCTGGAATGAT | 58 | 133 |
| downstream: ATGGCAGACTGCTGAAGGTC | ||||
| GnRH Receptor | KJ659046.1 | upstream: TCTGCTGGACCCCCTACTAC | 60 | 127 |
| downstream: TCCAGGCAGGCATTGAAGAG | ||||
| GnIH Receptor | KF696709.1 | upstream: GTCGTCATGTACACCCGCAT | 56 | 103 |
| downstream: TCTTGCGAGACACCTTCCTC | ||||
| VIP Receptor-I | NM_001097523.1 | upstream: TACTGCGTCATGGCCAACTT | 58 | 153 |
| downstream: TGTCCAAGCGGTGATGAACA | ||||
| TRH Receptor | NM_204930.1 | upstream: CTATGGCTATGTGGGGTGCC | 60 | 189 |
| downstream: ACTGAGGCGAAAGACCAGAC | ||||
| FSH beta | EU252532 | upstream: GTGGTGCTCAGGATACTGCTTCA | 60 | 209 |
| downstream: GTGCAGTTCAGTGCTATCAGTGTCA | ||||
| LH beta | DQ023159 | upstream: GACCCGGGAACCGGTGTA | 58 | 90 |
| downstream: AGCAGCCACCGCTCGTAG | ||||
| PRL | DQ023160 | upstream: TGCTCAGGGTCGGGGTTTCA | 56 | 218 |
| downstream: GCTTGGAGTCCTCATCGGCAAGTT | ||||
| TSH beta | FJ797681.1 | upstream: CTCTGTCCCAAAACGTGTGC | 56 | 121 |
| downstream: CCACACTTGCAGCTTATGGC | ||||
| LH Receptor | XM_013192443.1 | upstream: CGGATACACAACGATGCCCT | 60 | 74 |
| downstream: GACTCCAGTGCCGTTGAAGA | ||||
| FSH Receptor | KC477215.1 | upstream: CCTAGCCATTGCTGTGCATTT | 60 | 106 |
| downstream: TGCCAGGTTGCTCATCAAGG | ||||
| 3-beta HSD | KC310447.1 | upstream: TGTGACGTTCCTGTACCGTG | 153 | |
| downstream: TTACAACGGGTACACGCCTC | ||||
| StAR | KF958133.1 | upstream: GGAGCAGATGGGAGACTGGA | 56 | 177 |
| downstream: CGCCTTCTCGTGGGTGAT |