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Live/dead staining of C. crescentus CB15 biofilm cells. The BacLight LIVE/DEAD viability kit was used to label biofilm cells. Green fluorescence indicates viable cells, and red fluorescence indicates dead cells. Biofilms were grown in xylose-minimal medium under flow conditions (200 μl/min). Images were taken 48 h (A) and 96 h (B and C) after inoculation.
