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Clonal growth of C. crescentus CB15 microcolonies. Experiments were initiated by seeding biofilm chambers with a 1:1 mixture of gfp- and dsred-tagged C. crescentus wild-type cells (AS110 and AS109). Biofilms were irrigated at a flow rate of 200 μl/min in xylose-minimal medium at 30°C. Images were recorded after 96 h.
