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. 2004 Dec;186(24):8193–8206. doi: 10.1128/JB.186.24.8193-8206.2004

FIG. 5.

FIG. 5.

RT-PCR analysis of RNA extracted from S. gordonii Challis 2 and nosX::Tn917-lac strains. (A) The organization of nos and adjacent genes, location of primers, and predicted size of successfully amplified RT-PCR products are shown. See Table 1 for all primers used. The black arrow denotes the position of the Tn917-lac insertion in the biofilm-defective mutant. (B) RT-PCR analysis of transcripts of the ORFs within the putative nos operon. The total RNA extracted from the Challis 2, nosX::Tn917-lac, nosX::Kanr, and qor1::Kanr strains was used as the template. Challis 2 DNA was used as the positive control to verify the fidelity of the primers used. Lanes: 1, 1-kb DNA marker; 2, Challis 2 DNA with primers O2P1 and O2P2 (spans nosX-qor2; 1,018 bp); 3, Challis 2 RNA with primers O2P1 and O2P2; 4, Challis 2 DNA with primers O1P3 and O1P4 (spans qor1-qor2; 858 bp); 5, Challis 2 RNA with primers O1P3 and O1P4; 6, nosX::Kanr RNA with primers O1P3 and O1P4; 7, nosX::Tn917-lac RNA with primers O1P3 and O1P4; 8, Challis 2 DNA with primers O2RT5′ and O2RT3′ (within qor2; 111 bp); 9, Challis 2 RNA with primers O2RT5′ and O2RT3′; 10, qor1::Kanr RNA with primers O2RT5′ and O2RT3′; 11, nosX::Tn917-lac RNA with primers O2RT5′ and O2RT3′.