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. 2004 Dec;186(24):8193–8206. doi: 10.1128/JB.186.24.8193-8206.2004

FIG. 6.

FIG. 6.

Effect of different growth conditions on nosX expression. The biofim-deficient nosX::Tn917-lac mutant was grown at 37°C under anaerobic conditions unless otherwise specified. The β-galactosidase activities of the nosX-lacZ transcriptional fusion were quantified with MUG (28). All assays were performed in triplicate, and mean values and standard deviations of the β-galactosidase activities are shown. MU, 4-methyl-umbelliferone. (A) Relation between growth phase and nosX expression. The nosX::Tn917-lac mutant was grown in THBYE. Growth and β-galactosidase activity at various times over 24 h are shown. (B) Effects of different concentrations of various substrates on nosX expression. The nosX::Tn917-lac mutant was grown in BM with different substrates or under static anaerobic conditions as indicated.