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6-P-gluconolactonase assays of ybhE/pgl mutant and wild type E. coli, and of bacteria containing the YbhE/Pgl expression vector and control plasmid. (A) ▵, Assay of MG1655 containing an intact ybhE/pgl gene; ○ and •, M2523 (ybhE<>amp) and M1971(ybhE<>tet), respectively. (B) •, M2524 (ybhE<>tet [pJL6]); ○, M2526 (ybhE<>tet with ybhE expression plasmid [pJL6-ybhE/pgl]); ▴, M2527 (cI857 [pJL6]); ▵, M2528 (cI857 [pJL6-ybhE/pgl]). Details of the assay are described in Materials and Methods.
