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DNase I footprinting assay. Probe A was incubated with various amounts of the purified CpxR (lane 1, 0 pmol; lane 2, 10 pmol; lane 3, 20 pmol; lane 4, 30 pmol; lane 5, 40 pmol; lane 6, 50 pmol; lane 7, 60 pmol; lane 8, 70 pmol; lane 9, 80 pmol) and subjected to DNase I footprinting assays. Lanes AG represent the Maxam-Gilbert sequence ladder. The black boxes and bold arrows indicate the CpxR binding region and the direct repeat, respectively.
