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Inhibition of Pu by glucose in P. putida MAD2 lacking the crc or cyoB gene. The Pu-lacZ reporter strain P. putida MAD2 and its crc::tet and cyoB::tet derivatives were subjected to a standard carbon (glucose, +G) Pu inhibition test. Note the partial relief of Pu downregulation in the crc mutant, which likely resulted from stimulation of the ED pathway for glucose metabolism (see the text for an explanation). β-gal, β-galactosidase; U. Miller, Miller units; wt, wild type.
