Table 1.
Primary Antibodies Used
| Antigen | Description of immunogen | Source, host species, catalog No., clone or lot, RRID | Concentration used | Characterization |
|---|---|---|---|---|
| PV (parvalbumin) | Rat muscle parvalbumin | SWant, rabbit polyclonal, PV 25, AB_10000344 | 1:2,000 (IHC) | Bunce et al., 2013; Cai et al., 2013 |
| SMI‐32 | Homogenized rat hypothalamii; subsequently determined to recognize nonphosphorylated epitopes on the neurofilament heavy chain | Covance Research Products Inc., mouse monoclonal, SMI‐32R‐500, AB_509998 | 1 µg/ml (IHC) | Brown and Sawchenko, 2007; Stillman et al., 2009 |
| NeuN | Purified cell nuclei from mouse brain | Millipore, mouse monoclonal, MAB377, clone A60, AB_10048713 | 1 µg/ml (IHC) | Wang et al., 2006; Eskilsson et al., 2014 |
| NF‐160 | Recombinant C‐terminal fragment of the rat medium neurofilament subunit | Abcam, rabbit polyclonal, ab9034, AB_306956 | 1:1,000 (IHC) | (Deng et al., 2009; tegeman et al., 2013 |