Figure 2.

Transient increase in IFN‐γ expression by CD4⁺ spleen cells from PD‐1 antibody‐treated mice bearing premalignant oral lesions. Mice bearing 4NQO‐induced premalignant oral lesions were treated with either isotype control or PD‐1 antibodies. After 1, 3 and 5 weeks of treatment, sample mice were sacrificed and their spleens were immunostained extracellularly for CD4 and intracellularly for IFN‐γ. Shown are representative fluorescent dot‐blots of spleen cells from mice treated with isotype control antibody or anti‐PD‐1 antibody.