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. 2016 Dec 8;16(4):348–359. doi: 10.1080/15384101.2016.1260210

Figure 5.

Figure 5.

RIPK1-related NF-κB signaling pathway was regulated by Xbp1s. (A) Western blotting analysis for RIPK1 and phosphorylation of P65 subunit of NF-κB in NCMs in the presence or absence of AngII (10−5M for 24h, Top) or ISO (10−5M for 24h, Bottom) after transfected with of si-Con or siXbp1. n = 3. *, ** indicates P<0.05 vs. si-Con, # indicates P<0.05 vs. si-Con+AngII or si-Con+ISO. (B) Western blotting analysis for ATF4 and ATF6 in NCMs treated with AngII (10−5M for 24 h, Top) or ISO (10−5M for 24h, Bottom) after transfected with siNOX4. n = 4. * indicates P<0.05 vs. si-Con+AngII. (C) Western blotting analysis for ATF4 in NCMs transfected with si-Con or siATF4(1–3). n = 3. * indicates P<0.05 vs. si-Con group. (D) Western blotting analysis for RIPK1 and phosphorylation of P65 subunit of NF-κB of in NCMs in the presence or absence of AngII (10−5M for 24h, Top) or ISO (10−5M for 24h, Bottom) after transfected with of si-Con or siATF4. n = 3. * indicates P<0.05 vs. si-Con group.