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. 2016 Nov 28;16(4):319–334. doi: 10.1080/15384101.2016.1259037

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Figure 5. — Constitutive MYC expression dismantles STAT3/SMAD3-induced senescence and cooperates with OSM to drive EMT. (A) Soft agar culture of shp53/MYC-HMEC plated in the presence or absence of recombinant OSM and recombinant TGF-β1 for 2 weeks. AIG was quantified by brightfield microscopy using Metamorph software. (B) Single-gene qRT-PCR analysis using primers targeting E-cadherin and Snail (encoded by CDH1 and SNAI1, respectively) and mRNA from shp53-HMEC and shp53/MYC-HMEC plated in the presence (+) or absence (-) of recombinant OSM and TGF-β1 for 7 d. (C) Western analysis of whole cell lysates from shp53/MYC-HMEC left untreated or treated with recombinant OSM for 7 d.