Figure 3.

Effect of PADI6 depletion on MSY2 localization in the insoluble fraction of the Padi6 KO oocytes. (A) Western blot showing that Triton X-100 extraction released MSY2 from the insoluble fraction in Padi6 KO oocytes, with actin as a loading control. Histograms represent normalized band densitometry readings averaged from 3 independent experiments. *p < 0.05. (B) Representative confocal images showing the mislocalization of MSY2 in Padi6 KO oocytes after triton extraction. Scale bar, 10 μm.