Figure 4.

Transcriptome analyses of miR-221 and miR-222 knockout cells. (A-C) Based on the RNA-seq data from wild type and knockout cells, we selected 5,605 mRNAs (see Materials and Methods). Among these mRNAs, we identified those with 3′ UTR containing the 7-mer motif common to miR-221–3p and miR-222–3p. The fold changes between 7-mer containing mRNAs and the other mRNAs were compared by cumulative plots. The levels of mRNAs from (A) 221KO, (B) 222KO, and (C) DKO cells were compared with those of wild type cells for the calculation of fold changes. The P values were calculated by the Kolmogorov–Smirnov test. (D-F) The fold changes of mRNAs between knockout cells were compared. The correlation values for each comparison were shown.