Figure 5.

Efm2 and its N‐terminal truncation mutants form oligomeric states. Wild‐type (WT) Efm2 and its N‐terminal truncation mutants were incubated with or without SDS in the presence of the crosslinker dimethyl pimelimidate. An anti‐PentaHis immunoblot was used to detect all relevant species. (A) Time‐series crosslinking analysis of wild‐type Efm2 indicates that it forms oligomeric states. (B) Crosslinking of Efm2 N‐terminal truncation mutants for 2 h. SDS (1%) added prior to crosslinking was used as a negative control for all N‐terminal truncations.