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. 2017 Feb;23(2):220–231. doi: 10.3201/eid2302.161072

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Binding of influenza A virus hemagglutinins to A) fetuin and B) transferrin. Limiting dilutions of soluble H5 trimers complexed with horseradish peroxidase−conjugated antibodies were used in a fetuin- or transferrin-binding assay. Optical density at 450 nm (OD₄₅₀) corresponds to binding of HA to glycoproteins. HA, hemagglutinin; H5N1_2.3.4, novel H5N1 virus clade 2.3.4; H5N1₁, H5N1 virus clade 1.