Figure 4.

Analysis of binding of influenza A virus H5N1_2.3.4 and H5N8 hemagglutinins to sialylated glycans by using biolayer interferometry. A) 3′SLN, B) 3′SLe^X, C) 3′SLNLN, D) 6′SLNLN. After complexing biotinylated glycans with streptavidin sensors, association and subsequent dissociation of H5 proteins complexed with StrepMAB-classic (IBA GmbH, Göttingen, Germany) was determined. Blue squares, N-acetylglucosamine; yellow circles, galactose; purple diamonds, sialic acid; red triangles, fucose. The dotted lines at the 20-min time points distinguish the association and dissociation phases. H5N1_2.3.4, novel H5N1 virus clade 2.3.4.