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. 2017 Feb;23(2):296–299. doi: 10.3201/eid2302.161432

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Phylogenetic tree of Zika virus partial nonstructural protein 5 gene of 3 strains detected from humans in Cambodia. The partial sequences of the nonstructural protein 5 gene (192–194 nt) generated from the PCR products obtained for each strain were analyzed and assembled by using CLC Main Workbench 5.5 package (CLC bio A/S, Aarhus, Denmark). MEGA6 (14) was used to perform multiple sequence alignment of Cambodia strains with Zika virus reference strains from Africa and Asia genotypes available in GenBank and phylogenetic analyses by using maximum-likelihood method using the general time reversible model with 1,000 bootstrap resampling. Spondweni virus (GenBank accession no. AF013406) was used to root the tree. Cambodia strains from this study are indicated by bold type and a black triangle, and the Cambodia strain isolated in 2010 is indicated by bold type and a white triangle. Scale bar indicates nucleotide substitutions per site.