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. 2017 Jan 19;13(2):322–332. doi: 10.1080/15548627.2016.1265192

Figure 1.

Figure 1.

FAM134B restricts DENV and ZIKV infection. (A and C) HBMEC transfected with the indicated siRNAs were infected with (A) DENV or (C) ZIKV (MOI of 1) for 24 h. Infection and host gene expression were determined by RT-qPCR. Data are presented as the fold change of the control (CONsi). (B and D) Extracellular (B) DENV and (D) ZIKV titer from HBMEC transfected with CONsi or FAM134Bsi, determined by fluorescent focus assay, data are presented as the focus-forming units per mL. (E) HBMEC transfected with CONsi and FAM134Bsi were infected with VSV expressing a GFP reporter (MOI of 1) for 7-8 h. Infection was determined by immunofluorescence (VSV). Data are presented as the fold change of CONsi. (F) HeLa cells stably propagating a DENV subgenomic replicon (DENVrep) were transfected with CONsi and FAM134Bsi. Replicon RNA and host gene expression were assessed at 48 h post transfection by RT-qPCR. Data are presented as the fold change of CONsi. All data represent the mean ± SD of at least 3 independent experiments. Significance was determined by Student t test, *p < 0.05 and ***p < 0.0005.