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. 2017 Jan 19;13(2):322–332. doi: 10.1080/15548627.2016.1265192

Figure 4.

Figure 4.

The FAM134B C-terminal fragment is unable to mediate reticulophagy. (A) Representative confocal images of U2OS cells transfected with a plasmid encoding mRFP-LC3B and GFP or GFP tagged FAM134B construct, and DAPI-stained nuclei. Insets show zoomed in region outlined by white boxes. White arrowheads point to mRFP- and GFP-positive puncta. Scale bars: 20 µm. (B) Quantification of the number of mRFP-LC3B and GFP fusion protein co-expressing cells with mRFP- and GFP-positive puncta. Data are presented as a percentage and represent the average ± SD of 3 independent experiments (≥ 100 cells analyzed/experiment). Significance was determined using the Student t test, ***p <0.0001.