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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Circ Heart Fail. 2017 Feb;10(2):e003658. doi: 10.1161/CIRCHEARTFAILURE.116.003658

Figure 5. H-Ras activates the PI3K-AKT signaling axis and promotes cardiomyocyte hypertrophy.

Figure 5

A and B. Ventricular homogenates from control and mutant mice were subjected to western blot analysis to examine downstream signaling in response to 4 week TAC. C. NRCMs were treated with control siRNA (si-CTRL), or siRNA targeted to H-Ras (si-Hras) or K-Ras (si-Kras) for 72 hours. Cells were then stimulated with phenylephrine (PE; 100 μM) or vehicle control for 1 hour. NRCMs were transduced with LacZ or H-Ras12V in combination with vehicle or inhibitor for 48 hours and qRT-PCR performed to detect Nppa (D), Nppb (E) and Myh7 (F). N=3–4. Data are mean ± SEM. *,P<0.05.