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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Am Chem Soc. 2017 Feb 7;139(6):2464–2467. doi: 10.1021/jacs.6b12731

Figure 3.

Figure 3

Streptavidin gel shifts characterizing extent of UBP retention in vivo. The recovered plasmids are PCR amplified with a dNaMTP analog that is biotin tagged and thus the percentage of the slower migrating (upper) band, relative to the sum of the slower and faster migrating bands represents the percent UBP retention. Note that the faster migrating (unshifted) band sometimes appears as a doublet, but this is observed with the input plasmid, as well, and thus is not related to in vivo replication. The percentage shifted is normalized to that measured for the input plasmid (shown on left). A molecular weight marker is included in the lane labeled “M.” See Supporting Information for details.