Figure 4.

CRKL is a direct target of miR-320 in gastric cancer cells.

Notes: (A) Luciferase reporter plasmid with wild-type and mutant binding site was transfected into SGC-7901 cells with miR-320 mimic. When transfected with wild-type reporter, cells showed a lower luciferase activity after transfection with miR-320 mimic. When transfected with mutant site reporter, there was no significant change in activity. (B) CRKL siRNA in SGC-7901 cells decreased protein levels of cyclin D1, MMP9. In CRKL-depleted cells, the inhibiting effect of miR-320 on cyclin D1 and MMP9 was not significant compared with cell without CRKL siRNA. (C) CRKL transfection restored MMP9 and cyclin D1 status in cells transfected with miR-320 mimic. *P<0.05.

Abbreviation: miR-320, microRNA-320.