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. 2017 Feb 20;10:1027–1038. doi: 10.2147/OTT.S125067

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© 2017 Zheng et al. This work is published and licensed by Dove Medical Press Limited

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miR-195 mediates radiosensitivity of CRC cells.

Notes: HCT-116 and HT-29 cells were transfected with miR-control (miR-con) or miR-195 mimics at 24 h of incubation. Survival fractions were calculated in transfected HCT-116 (A) and HT-29 cells (B) after treatment with 0, 2, 4, 6, and 8 Gy of ionizing radiation for 24 h. Cell apoptosis was detected by flow cytometry in transfected HCT-116 (C and D) and HT-29 (E and F) cells at 24 h after treatment with or without ionizing radiation (4 Gy). FITC, fluorescein isothiocyanate. The levels of apoptosis-related proteins Bax, Bcl-2, and γ-H2AX were measured by Western blot in transfected HCT-116 (G) and HT-29 cells (H) after treatment with or without ionizing radiation (4 Gy) for 24 h. *P<0.05 vs miR-control.

Abbreviations: NC, negative control; PI, propidium iodium; FITC, fluorescein isothiocyanate; CRC, colorectal cancer.

miR-195 mediates radiosensitivity of CRC cells.

Notes: HCT-116 and HT-29 cells were transfected with miR-control (miR-con) or miR-195 mimics at 24 h of incubation. Survival fractions were calculated in transfected HCT-116 (A) and HT-29 cells (B) after treatment with 0, 2, 4, 6, and 8 Gy of ionizing radiation for 24 h. Cell apoptosis was detected by flow cytometry in transfected HCT-116 (C and D) and HT-29 (E and F) cells at 24 h after treatment with or without ionizing radiation (4 Gy). FITC, fluorescein isothiocyanate. The levels of apoptosis-related proteins Bax, Bcl-2, and γ-H2AX were measured by Western blot in transfected HCT-116 (G) and HT-29 cells (H) after treatment with or without ionizing radiation (4 Gy) for 24 h. *P<0.05 vs miR-control.

Abbreviations: NC, negative control; PI, propidium iodium; FITC, fluorescein isothiocyanate; CRC, colorectal cancer.