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. 2017 Feb 20;10:1027–1038. doi: 10.2147/OTT.S125067

Figure 3.

Figure 3

Figure 3

Effect of CARM1 knockdown on the radiosensitivity of CRC cells.

Notes: HCT-116 and HT-29 cells were transfected with si-control (si-con) and si-CARM1 at 24 h of incubation. Survival fractions were calculated in transfected HCT-116 (A) and HT-29 cells (B) after treatment with 0, 2, 4, 6, or 8 Gy of ionizing radiation for 24 h. The CARM1 level was detected using Western blot in HCT-116 (C) and HT-29 cells (D) after treatment with or without ionizing radiation (4 Gy) for 24 h. Cell apoptosis was detected by flow cytometry in transfected HCT-116 (E) and HT-29 (F) cells after treatment with or without ionizing radiation (4 Gy) for 24 h. The levels of apoptosis-related proteins Bax, Bcl-2, and γ-H2AX were determined by Western blot in transfected HCT-116 (G) and HT-29 cells (H) after treatment with or without ionizing radiation (4 Gy) for 24 h. *P<0.05 vs si-control.

Abbreviations: NC, negative control; CRC, colorectal cancer.