Figure 2.

Tbx2 overexpression correlates with proliferation and invasion.

Notes: (A) Western blot and RT-PCR analysis showed that Tbx2 protein expression was high in U251 cell line and low in U87 and A172 cell lines. (B) Tbx2 siRNA treatment decreased its mRNA and protein expression in U251 cells. Tbx2 plasmid transfection upregulated its mRNA and protein levels. (C) CCK8 assay demonstrated that Tbx2 overexpression accelerated cell growth rate, while Tbx2 depletion inhibited cell proliferation. Cell growth rate was higher in U251 cell line than other cell lines. (D) Matrigel invasion assay showed that Tbx2 depletion decreased cell invasion in U251 cell line. Tbx2 plasmid transfection increased the number of invading cells. *P<0.05.

Abbreviations: RT-PCR, real-time polymerase chain reaction; CCK8, Cell Counting Kit-8.